Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences DuolinkR In Situ Detection
Duolink(R) In Situ Detection Reagents Green contains all the necessary Duolink In situ reagents to perform the amplification and detection of bound PLA(R) probes. The detection probes contain a fluorophore (lex 495 nm and lem 527 nm) which may be visualized using the same filter as Cy(R)2 or fITC. Experiments conducted using Duolink In situ reagents can detect and visualize protein interactions protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.
Sigma Aldrich Fine Chemicals Biosciences fastStartTM High fidelity
The fastStart(TM) High fidelity PCR System is a blend of fastStart Taq DNA Polymerase and a thermostable proofreading protein which is also chemically modified. This protein mediates proofreading activity but carries no polymerase activity. Both proteins are inactive below 75 C and are activated by heating to 95 C for two minutes.Hot start protocols have improved specificity sensitivity and PCR yield. Heat-labile blocking groups on amino acid residues of fastStart(TM) Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically. fastStart(TM) Taq DNA Polymerase is activated by removal of blocking groups using a preincubation step at 95C. The fastStart(TM) High fidelity PCR System is the product of choice for multiplex PCR. fastStart(TM) High fidelity Enzyme Blend (5 U/mul) in storage bufferfastStart(TM) High fidelity Reaction Buffer 10x concentrated with 18 mM MgCl2fastStart(TM) High
Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 fI Assa10k
The Transcreener(R) HTS Assay platform overcomes the need for time-consuming one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener(R) HTS Assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 fluorescent intensity (fI) assay extends the Transcreener(R) platform for ADP detection by utilizing a simple fluorescent intensity output which can be used on both fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers more commonly used in core facilities and HTS labs. The Transcreener(R) ADP2 fI Assay is a red competitive fluorescence intensity (fI) assay based on the detection of ADP and therefore is compatible with any enzyme class
Sigma Aldrich Fine Chemicals Biosciences AccumaxTM solutin sterile500ML
AccumaxTM SOLUTIONn sterile500ML
Sigma Aldrich Fine Chemicals Biosciences PYREXR Disposable Glass Co
These disposable 50mL Pyrex glass centrifuge tubes are manufactured from Type I borosilicate glass and have a GPI 24-400 thread. They are supplied without caps. The tubes are designed to withstand centrifugation up to 3 000 RCf and are packed in convenient easy to use packs which are shrink-sealed for protection during shipment. for reusable phenolic screw caps with white rubber liners see CLS999924 for reusable phenolic screw caps with Polytetrafluorethylene (PTfE) faced rubber liners see CLS999824.
Sigma Aldrich Fine Chemicals Biosciences fORTUNAR OptifixR Solvent1EA
for dispensing solvents featuresDispensing piston made of glassVisible dispensing process by the use of a dispensing cylinder made of glass coated with plastic-protection against breakagefIX adapter made of white PP up to 10 mL fIX adapter made of solid white PP from 30 mL sizeValve block made of PTfE no metal springs in the valve - no corrosion Ergonomic finger protection capfast and easy volume setting with additional fine adjustmentOptifix Solvent dispenser available up to 500 mLSupplied as Optifix Solvent dispenser 2 PP adapters GL40 GL45 1 PTfE intake tube
Sigma Aldrich Fine Chemicals Biosciences CfTM 633 succinimidyl este
far-red fluorescent dyes offer the advantage of ultra sensitive detection because background signal due to autofluorescence in most biological samples is minimal in this spectral region. for many years the cyanine dye Cy(R)5 has been the dye of choice for such detection. More recently Alexa fluor(R) 647 has been developed as a better alternative by having brighter fluorescence and higher photostability. Despite the improvement Alexa fluor(R) 647 still lacks sufficient photostability required for many demanding applications. On the other hand while Alexa fluor(R) 633 is photostable its fluorescence on proteins is very weak. In fact it has been a challenging task for dye chemists to develop a far-red fluorescent dye that is both highly fluorescent and photostable for protein and nucleic acid labeling. Using new chemistry scientists at Biotium have successfully developed a series of far-red and near-IR dyes to overcome these challenges. With its absorption peak at 630 nm Cf(TM)633
Sigma Aldrich Fine Chemicals Biosciences 3 Mercaptopropyl functiona100G
3 Mercaptopropyl functiona100G
Sigma Aldrich Fine Chemicals Biosciences Brequinar sodium salt hydr25MG
Brequinar sodium salt hydr25MG
Sigma Aldrich Fine Chemicals Biosciences Larazotide acetate salt 9525MG
Larazotide acetate salt 9525MG
Sigma Aldrich Fine Chemicals Biosciences Omnipore Membrane filter 1
Our fluoropore(R) membrane is a hydrophobic polytetrafluoroethylene (PTfE) polymer membrane bonded to a high density polyethylene support to improve the handling characteristics of the filter for normal applications. fluoropore membranes provide broad chemical compatibility high flow rates and consistency. (Please note that catalogue numbers containing fHUP are unlaminated or produced without a backing.)Our Omnipore membrane is a hydrophilic PTfE membrane that is compatible with virually all solvents acids and alkaline SOLUTIONons.features Benefits - Biologically and chemically inert - High porosity yields high flow rates - Hydrophobic and hydrophilic PTfE varieties available for filtration of aqueous and organic-based samples Applications - fluoropore Membrane Clarifying Acids Bases and Solvents Air Monitoring filtering and Venting Gases UV Spectroscopy - Omnipore Membrane filtration of Aqueous SOLUTIONons Clarifying Acids and Alkaline SOLUTIONon
Sigma Aldrich Fine Chemicals Biosciences 4-Vinylphenol solution 10100G
4-Vinylphenol SOLUTIONon has been used as an HPLC internal standard during the identification of 4-vinyl derivatives formed via decarboxylation of hydroxycinnamic acids extracted from corn cobs using phenolic acid decarboxylase (PAD) enzyme extracted from Lactobacillus plantarum.
Sigma Aldrich Fine Chemicals Biosciences PolyDL lactide co glycolid5G
The product is an amorphous solid with a transition temperature (Tg) in the range of 45-50C. It is soluble in most organic solvents such as methylene chloride tetrahydrofuran ethyl acetate acetone chloroform and hexafluoroispropanol.